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Author: DaanJansen94

README.md

@@ -17,7 +17,7 @@ RecMpox is a command-line tool that **flags potential recombination events** in
    - Interactive HTML report: Provides sortable tables, summary plots, per-sample visualisations, and genome-wide displays of inferred recombination tracts and breakpoints.
    - With `-phylogeny`: Phylogeny folder with alignment, midpoint-rooted tree, and PDF/SVG figures; the tree is also included in the HTML report.
 
-⚠️ **Note**: RecMpox is primarily designed to investigate potential recombination between viruses circulating in sustained human outbreaks (for example, SH2017, SH2023b, and SH2024a). The reference genomes provided by default in the tool correspond to these sustained outbreak lineages. When applying RecMpox outside this context, select reference genomes that are genetically close to your consensus sequences, or alternatively use the optional **-phylogeny** mode to confirm that the two tracts cluster with two different outbreaks.
+⚠️ **Note**: RecMpox is primarily designed to investigate potential recombination between viruses circulating in sustained human outbreaks (for example, SH2017, SH2023b, and SH2024a). Outside this context, the most robust strategy is to combine MDRF with phylogeny to reduce false positives: elevated MDRF can reflect ancestral structures rather than true recombination, but if both tracts cluster within the same outbreak in the phylogeny, recombination can be excluded. Alternatively, select reference genomes that are genetically close to your consensus sequences.
 
 🔴 **Caution — Intra-clade comparisons**: When comparing within a clade, set the threshold higher than the default. Intra-clade comparisons yield far fewer diagnostic SNPs (e.g. as low as ~120 between Ia and Ib), meaning each individual SNP carries more weight and small percentages can arise from convergent evolution rather than true recombination. We recommend a minimum threshold of **20%** (`-m 20`) for intra-clade recombinant screening.