Skip to content

Commit 44229d5

Browse files
committed
docs initial commit
1 parent c2f1e0c commit 44229d5

6 files changed

Lines changed: 9861 additions & 0 deletions

File tree

docs/index.md

Lines changed: 11 additions & 0 deletions
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,11 @@
1+
# Cytovanni
2+
3+
Computational Standardization of Flow Cytometry Data
4+
5+
---
6+
7+
## Tutorials
8+
9+
The first tutorial shows how to use [rainbow beads to standardize the flow cytometer](tutorials/integration_fixed-spectra/integration_fixed-spectra-rainbow.ipynb), and [fit scaling factors for each marker using reference samples](tutorials/integration_fixed-spectra/integration_fixed-spectra-ref.ipynb). While we strongly encourage the use of rainbow beads, the scaling factors can also be used independently from the cytometer standardization, and will be the dominant source of batch effects in many setups.
10+
11+
The second tutorial also uses the [rainbow bead standardization](tutorials/integration_variable-spectra/integration_variable-spectra-rainbow.ipynb), which is applied to the samples, as well as to single stain spectra. We then [model the dye variability](tutorials/integration_variable-spectra/integration_variable-spectra-dye.ipynb), which allows us to [fit a full integration from overlapping samples](tutorials/integration_variable-spectra/integration_variable-spectra-model.ipynb) including both marker scaling factors, as well as to extract dye spectra for every batch from the overlapping samples.

0 commit comments

Comments
 (0)