scrna_seurat.md

Single-cell RNA-seq Analysis (Seurat)

This module provides functionality for analyzing single-cell RNA sequencing data using the Seurat package in R.

Overview

The scRNA-seq analysis with Seurat includes:

1. Data loading and preprocessing
2. Normalization and feature selection
3. Dimensionality reduction (PCA)
4. Clustering
5. Visualization (UMAP)

Main Functions

• process_scrna_seurat(data_path): Processes scRNA-seq data using Seurat.

Usage

from src.scrna_seurat.scrna_seurat import main

main('path/to/your/10x/data')

This will process the scRNA-seq data using Seurat and output the results.