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Samplesheets

These files are example CSV samplesheets. Each has a header row and one row per sample:

Column Meaning
sample Sample name used in the pipeline.
barcode Depends on the file (see below).

fastq.csv — for runs that start from FASTQ reads: barcode is the full path to that sample’s read file. To auto-generate a samplesheet CSV from a folder of FASTQ files, follow the --input note in all_options.md.

POD5.csv — use this samplesheet when starting from raw signal (pod5) data, or from basecalled but not demultiplexed reads (fastq_pass). In both cases, barcode is the barcode label (e.g. barcode01) for that sample. The data directory is set separately in your params file (input_dir), not in the CSV.

all_options.md — pipeline parameters as tables.