Skip to content

Folders and files

NameName
Last commit message
Last commit date

Latest commit

 

History

5 Commits
 
 
 
 
 
 

Repository files navigation

These scripts run all the steps needed to:

  1. Quality trim and filter transcriptome reads with trim_galore,
  2. Identify repeats in your genome de novo with RepeatModeler,
  3. Mask repeats with RepeatMasker,
  4. Run ProtHint to generate hints from translated transcriptomes (or do this as part of the BRAKER3 pipeline),
  5. Map trimmed/filtered reads to the genome with STAR/HISAT2,
  6. Run BRAKER to annotate the genome

Some relevant reading: https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ http://www.repeatmasker.org/RepeatModeler/ http://www.repeatmasker.org/RepeatMasker/ https://www.biostars.org/p/411101/ https://github.com/gatech-genemark/ProtHint https://github.com/Gaius-Augustus/BRAKER/ https://github.com/Gaius-Augustus/TSEBRA

Note that these scripts are written for Kevin Kocot's workstation and some paths are hard-coded (search for "wirenia" to find them)

About

Scripts to streamline annotating a genome

Resources

Stars

1 star

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages